Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium.
نویسندگان
چکیده
منابع مشابه
Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium.
Madin-Darby canine kidney (MDCK) cells can grow in synthetic medium supplemented with five factors--insulin, transferrin, prostaglandin E1, hydrocortisone, and triiodothyronine--as a serum substitute. These five factors permit growth for 1 month in the absence of serum and a growth rate equivalent to that observed in serum-supplemented medium. Dibutyryl adenosine 3',5'-cyclic monophosphate subs...
متن کاملMadin-Darby Canine Kidney Epithelial Cell Line
The Madin-Darby canine kidney (MDCK) cell line, derived from distal tubule/ collecting duct, expresses differentiated properties of renal tubule epithelium in culture. We studied the expression of adrenergic receptors in MDCK to examine the role of catecholamines in the regulation of renal function. Radioligand-binding studies demonstrated, on the basis of receptor affinities of subtype-selecti...
متن کاملCharacterization of insulin-like growth factor I receptors on Madin-Darby canine kidney (MDCK) cell line.
Specific insulin-like growth factor I (IGF-I) receptors on the Madin-Darby canine kidney (MDCK) cell line were identified and characterized. [125I]IGF-I specifically bound to the cells, but [125I]insulin bindings to the cells was minimal. Unlabeled IGF-I displaced both the IGF-I and insulin bindings with potencies that were 100 and 10 times as great as insulin. By an affinity labeling technique...
متن کامل(CFTR)-mediated chloride secretion in type I Madin- Darby canine kidney (MDCK) cell line
word count: 248 11 Corresponding Author: 12 Sunhapas Soodvilai, Ph.D. 13 Department of Physiology 14 Faculty of Science, Mahidol University 15 Rama VI Road, Bangkok 10400 Thailand 16 Phone number: +66-2-2015516 17 Fax number: +66-2-3547154 18 Email:[email protected] 19 Articles in PresS. Am J Physiol Renal Physiol (May 29, 2013). doi:10.1152/ajprenal.00579.2012 Copyright © 2013 by the ...
متن کاملRegulated Synthesis and Functions of Laminin 5 in Polarized Madin-Darby Canine Kidney (MDCK) Epithelial Cells
Renal tubular epithelial cells synthesize laminin 5 (LN5) during regeneration of the epithelium following ischemic injury. LN5 is a truncated laminin isoform of particular importance in the epidermis, but also constitutively expressed in a number of other epithelia. To investigate the role of LN5 in morphogenesis of a simple renal epithelium, we examined the synthesis and function of LN5 in the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1979
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.76.7.3338